PCR

PCR temperature gradient

Column temperatures from your primer Tm values, with a suggested range

Forward primer Reverse primer

—Forward primer Tm

—Reverse primer Tm

—Suggested range

First column temperature °C Last column temperature °C Number of columns Set the range from the Tm values

Temperatures are spread linearly between the two ends. The real gradient across an instrument block is not perfectly linear and varies between manufacturers; consult your own manual for the exact value of each column. Tm uses the nearest-neighbour model under typical PCR conditions.